Enter the factor, not the ratio: 1:1 with trypan blue = 2, a 1:10 sample dilution = 10, none = 1. Combined factor 2×
cells/mL = mean count per large square × dilution × 10⁴
Chamber depth assumed 0.1 mm, large square area 1 mm². Count 4–5 large squares; aim for 20–50 cells per square. Tap the grid to count, long-press to undo the last cell.